The lateral flow immuno assay is a relatively old diagnostic platform that was developed in the 1980s. This animation takes a look inside a lateral flow immunoassay lfia and looks at the various components used to see how they interact with one another to produce rapid tests results.
Thermo scientific prospect range are enzyme immunoassays eia in a familiar microplate format giving sensitive and specific results.
Lateral flow immunoassay. Lateral flow tests also known as lateral flow immunochromatographic assays or rapid tests are simple devices intended to detect the presence of a target substance in a liquid sample without the need for specialized and costly equipment these tests are widely used in medical diagnostics for home testing point of care testing or laboratory use. A sample pad which is the area on which sample is dropped. A lateral flow assay lfa is composed of four parts.
However there are different immunoassay formats available. Basically it is a simple to use diagnostic device used to confirm the presence or absence of a target analyte such as pathogens or biomarkers in humans or animals or contaminants in water supplies foodstuffs or animal feeds. And absorbent pad which reserves waste.
Here we report an amplification free nucleic acid immunoassay implemented on a lateral flow strip for the fluorescence detection of severe acute respiratory syndrome coronavirus 2 sars cov 2 rna in less than one hour. Conjugate pad on which labeled tags combined with biorecognition elements. Preceding research has focused on the development of nanoparticles that were used in e g agglutination assays gold and on the design of new assay principles the flow through and the lateral flow technologies.
Lateral flow immunoassay covers the scope of utilization the principle of the technology the patent concerns information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the gereal scientific community. Dna rna double stranded hybrids and enables the conversion of nucleic acid testing into immunofluorescence on a. Rapid lateral flow immunoassay enables fluorescence detection of sars cov 2 rna.
For instance the home pregnancy test is a. So what is a lateral flow immunoassay. Choose the format to suit your laboratory workflow whether you are looking for enzyme immunoassays for high throughput or lateral flow immunoassays for convenient out of hours testing or low testing volumes.
We have chosen to explain how a multiplex sandwich immunoassay format works using a generic target sample. Reaction membrane containing test line and control line for target dna probe dna hybridization or antigen antibody interaction.